Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens.

Directly from Biopsy Specimens Helicobacter pylori and Genotyping of Multiplex PCR Assay for Rapid Detection

A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex-Urease PCR Results with Rapid Urease Test and Histopathology

Purpose: To develop a multiplex-urease PCR assay specific for ureA and ureB genes directly from gastric biopsy specimens and compare the results of multiplex-urease PCR assay with rapid urease test (RUT) and histopathology. Methodology: This study was conducted on 109 patients. A RUT was run; histopathological staining and multiplex urease PCR were applied to biopsy specimen to detect H. pylori...

Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method

In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...

Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis

Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. Existing detection methods are low-throughput, time-consuming and labor intensive. Therefore, a rapid and high-throughput meth...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

Helicobacter pylori is the causative agent of chronic gastritis and peptic ulcer diseases and is also a risk factor for gastric cancer. Since culture of Helicobacter pylori is relatively insensitive and cumbersome, PCR-based molecular detection and typing of this organism is gaining importance for strain differentiation. The aim of present study was to apply molecular methods for detection and ...